Ahr^em1Bra

CRISPR/Cas9-targeting in Ahrtm3.1Bra zygotes induced a single nucleotide change. Residue 375 was changed from a valine to an alanine (p.V375A) to change the low-affinity d allele (as found in the 129 strain) to the high-affinity b allele (as found i the B6 strain). There is also a silent mutation downstream. The genetic code (with the mutation denoted by upper case) reads: gGcgatgatAtaat which was changed from gAcgatgatGtaat.