在14-16号外显子的两侧,我们插入了loxP位点。同时,一段由loxP位点分隔的EGFP和诺卡因筛选元件,再以FRT位点包裹,被插入在14-16号可删除外显子上游。通过FLP介导的重组,去除了FRT标记的EGFP和诺卡因筛选组件,留下一个FRT位点和保留的14-16号可删除外显子。(来源:J:276323)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入
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1
5
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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