在exon 2的上游插入了一个FRT-loxP-neo-FRT-LoxP的 cassette,然后在exon 5和6之间插入了一个loxP位点。通过FLP介导的重组,去除了含Frt标签的neomycin cassette,留下loxP位点。(来源:J:284998)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count