基因的2-6外显子被LoxP位点前后包覆,接着插入了一个由腺病毒转录起始子和GFP基因构成的 cassette,后者位于第二个LoxP位点下游。通过FLP介导的重组,去除了含Neo抗性基因的弗里特-flanked序列。当Cre介导的重组发生时,2-6外显子被剔除,同时GFP报告基因在内源性启动子驱动下表达。(来源:J:262898)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6.Cg-Thy1a
Targeted
插入
--
1
4
8

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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