设计了四种寡核苷酸引导RNA(ACAAAATCTACCCTCTAGTA、GTCAGACACTGCCGTACTAG、TAAAGTTGTAGATAGACACT和AGACACTGGGACAAGCTTGG),通过CRISPR/Cas9基因编辑技术,旨在产生一个涉及1号基因外显子1/内含子1的大段框架插入突变。对后代的DNA测序结果显示,这一等位基因是一个897个核苷酸的框架插入,从3'端的ATG启动密码子开始,延伸至内含子1的100个碱基。来源:(J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ
Endonuclease-mediated
基因内删除
--
1
9
--

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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