Iqsec2^em1Frk

This CRISPR/Cas9 mediated deletion of a single nucleotide T at position 2579, in exon 7, results in a a phenylalanine to serine mutation at amino acid 860 which causes a frameshift mutation that introduces a stop codon. Semi-quantatative PCR and Western blot analysis indicate this is a null allele. (J:284762)