靶向载体被设计成插入一个HaloTag基因组序列(如下)、TEV酶识别序列、一个在第一个编码外显子(exon 1)上游的in-frame loxP-STOP-IRES-FLAG-loxP座,以及基因1的内含子1中的FRT引导的PGK-neo座。HaloTag是一个由罗氏罗德罗氏菌(DhaA基因)编码的33千道尔顿的卤代烷脱卤酶,它经过基因改造,能与合成的卤代烷配体形成不可逆共价键。通过Flp介导的重组,FRT引导的neo座被移除了。(来源:J:283893)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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