Ago2^{tm1.1(DhaA*)Aven}

The targeting vector is designed to insert a HaloTag cDNA sequence (see below), a TEV protease recognition sequence and an in-frame loxP-STOP-IRES-FLAG-loxP cassette upstream of the first coding exon (exon 1), and an FRT-flanked PGK-neo cassette in intron 1 of the gene. HaloTag is a 33 kDa haloalkane dehalogenase encoded by the DhaA gene from Rhodococcus rhodochrous that has been mutagenized to form an irreversible covalent bond to synthetic chloroalkane ligands. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:283893)