Hells^{tm1d(EUCOMM)Wtsi}

The L1L2_gt2 cassette was inserted at position 38939858 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 38940603. The critical exon(s) was thus flanked by loxP sites and a "conditional ready" (floxed) tm1c allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neomycin selection cassette, and subsequent breeding to a cre-deleter removed the critical exon(s) yielding this null allele. (J:283594)