The targeting construct inserted into the locus a CAGGS promoter (CAG), a floxed PGK-puromycin STOP cassette (LSL), the mouse Dnd1 cDNA with N-terminal FLAG and myc tags, and an frt site. Upon cre-mediated excision of the STOP cassette, the minigene constitutively expresses the FLAG/myc tagged Dnd1 driven by the CAG promoter. (J:281756)