靶向载体设计为在exon 2上游插入一个loxP位点,接着是FRT-PGK-neo-pA-SD-IS-FRT的元件,后面跟着exon 2下游的第二个loxP位点。SD是splice donor序列,IS是一个54bp的不稳定性信号。(来源:J:283234)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count