将靶向载体构建于Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze中,通过替换tdTomato元件,采用的是DR-SV-HRP,这是一种依赖于Cre和Flp双重重组酶的、专司突触囊泡(SV)的、马铃薯晚疫病酚氧化酶(HRP)报告基因元件。在小鼠Gt(ROSA)26Sor基因的第1内含子中插入了如下序列:CAG-FRT-Stop-FRT-loxP-Stop-loxP-SV(突触囊泡蛋白,SV导向肽)-HRP-HRP-WPRE(木瓜酵母病毒后转录调控元件)-bGHpA(牛生长激素polyA信号)-attB-PGK-NeoR-PGKpA-attP。来源:(J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129S4/SvJae
Targeted
插入
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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