通过基因合成和重组技术,构建了一个靶向构造。Cre编码序列和含Frt内切点的诺卡因(Neo)座片段被插入Robo3基因的第1外显子,以替换原有的起始翻译密码子及之后的31个核苷酸。随后,通过与FLPe小鼠杂交,删除了Neo座。(来源:J:282827)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6(Cg)-Tyrc-2J
Targeted
插入
--
1
1
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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