CRISPR/Cas9基因编辑技术用于将基因第15外显子的lysine(489)编码位置修改为arginine(AAA转为AGA)。这种突变产生了一个与裸鼹鼠(Heterocephalus glaber)种群中编码激酶域内早期出现的相似的早终止密码子。同时,为避免Cas9的PAM序列重定向,还插入了两个G到T的替换。(来源:J:280429)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
核苷酸替换
--
1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部