设计了一个质粒,携带有导向RNA和PAM序列TGG,目标是将P2A肽连接到一个cre/ERT2 cassette,插入SLC17A8基因(钠依赖性无机磷酸转运体8成员)的第12外显子,紧接在终止密码子之前。引导RNA和Cas9核酸酶通过在C57BL/6N来源的受精卵细胞质中导入,这些细胞核已经明确识别。(来源:J:282727)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N
Endonuclease-mediated
插入
--
1
2
5

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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