Mfsd1^{tm1c(KOMP)Wtsi}

The L1L2_Bact_P cassette was inserted at position 67492492 of Chromosome 3 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 67493280. The critical exon 3 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ sequence and created a conditional ready (floxed) allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:280713)