使用sgRNA5'-AGGTGGCTCTTGATGAGGCGGGG3'靶向CRISPR编辑,去除了3'端12个碱基,影响了His111活性位点周围的构象。 Western blot结果显示大脑中没有蛋白质表达(来源:J:280143)
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CRISPR-targeting of exon 3 using sgRNA 5'-AGGTGGCTCTTGATGAGGCGGGG3' deleted 12 bp, disrupting the structure around the active site His111. Western blot analysis confirmed the absence of protein expression in the brain. (J:280143)