CRISPR对exon 1剪切的靶点,删除了62个bp。 Western blot结果显示睾丸中没有蛋白表达,免疫荧光也证实了精子中没有蛋白表达。(来源:J:279929)
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CRISPR-targeting of exon 1 deleted 62 bp. Western blot analysis confirmed the absence of protein expression in testes. Immunofluorescence confirmed the absence of protein expression in sperm. (J:279929)