Col1a1^{tm3(tetO-H3f3a)Hoch}

A targeting vector was designed to insert an FRT-flanked PGK-Neo-pA 500 bp downstream of the 3' UTR of the Col1a1 locus. The FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus was replaced by co-injection of a tetO-H3.3f3a plasmid and flpe recombinase. The tetO-H3.3f3a plasmid contains a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of the H3.3 histone A gene. (J:341515)