Prph2^tm2.1Itl

Two point mutations were introduced to exon 2 of the gene. The first was a silent G>A to eliminate a Hha1 restriction site to aid in genotyping, and the second was the A>G missense mutation to produce the p.N229S substitution in the encoded protein. An FRT site flanked neomycin selection cassette was removed via flp-mediated recombination. (J:276288)