将loxP序列和Neo cassette,前后带有两个Frt座标,插入在第12外显子上游331bp的位置。接着,将小鼠Hprt基因的内源性核糖体进入位点(IRES)和剪接起始点(SD)序列插入Neo cassette,用于poly-A捕获策略。另一个loxP座标则被引入在第12外显子下游185bp的位置,目的是在Cre介导重组后消除潜在的跨膜域。(来源:J:279639)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N
Targeted
插入
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1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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