CRISPR/Cas9介导的突变产生了70bp的缺失,发生在exon 2。 Western blot结果显示,纯合突变体睾丸中没有蛋白质表达(来源:J:278620)
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CRISPR/Cas9 mediated mutagenesis created a 70-bp deletion in exon 2. Western blot analysis confirmed the absence of protein expression in homozygous mutant testes. (J:278620)