Left TAL effector (50,191,867-50,191,883 bp) and right TAL effector (50,191,899-50,191,915 bp) separated by the FokI cleavage zone is used to introduce a 16 bp frameshift deletion in the first coding exon (exon 4). The mutation results in a premature stop codon. Founder 28 is established. (J:232145)