Tg(Lepr-Bmncr)#Xhl

The transgenic vector was constructed by subcloning the mouse Bmncr cDNA into a plasmid containing the Lepr promoter. The vector was then purified and microinjected into C57BL/6J F2 mouse oocytes, and the oocytes were surgically transferred into pseudopregnant C57BL/6J dams. Two lines with higher levels of Bmncr expression were selected from 6 transgenic founders and bred in C57BL/6 strain for 6 generations to obtain offspring with a defined genetic background. One line with 10-fold overexpression of Bmncr was used for studies. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:270992)