将1-5号外显子替换为含弗里特(Frt)筛选标记的诺卡因选择座。来自纯合突变小鼠肝脏和肌肉组织的蛋白质提取物 Western blot 显示编码蛋白缺失。(来源:J:264446)
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Exons 1-5 were replaced with an frt-flanked neomycin selection cassette. Western blots of protein extracts from liver and muscle tissues derived from homozygous mutant mice demonstrated the absence of the encoded protein. (J:264446)