Exon 3被替换成一个PGK-诺卡因选择标记元件。通过半定量RT-PCR、 Western blotting和免疫荧光分析,确认了编码蛋白在RNA和蛋白质水平上没有表达(来源:J:278420)。
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Exon 3 was replaced with a PGK-neomycin selection cassette. The absence of the encoded protein expression was confirmed at both RNA and protein levels by semi-quantitative RT-PCR, Western blotting and IF analysis. (J:278420)