1-8号外显子被新霉素抗性基因座替换。RT-PCR和 Western blot 实验确认了成年纯合突变小鼠胰腺中没有转录物和编码蛋白的存在(来源:J:278344)
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Exons 1-8 were replaced by a neomycin selection cassette. RT-PCR and Western blot analysis confirmed the absence of transcripts and encoded protein in the pancreas of adult homozygous mutant mice. (J:278344)