在Furin上游,针对NKX2-5结合增强子M10设计了两个sgRNAs,目标序列分别为ATGCGGTTGTGATCTCTCGG和GCCACCGGCAAAGGCGTGGT,使用CRISPR/Cas9技术后,成功实现了676行的896bp缺失(来源:J:273415)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count