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中文
将1到5号外显子替换为一个含氨苄青霉素抗性基因的克隆载体。通过Cre介导的重组移除了这个neo cassette。这种方法产生了DMKNβ和γ亚型缺失的转基因小鼠，但能表达α亚型。通过RT-PCR分析确认了β和γ亚型的完全缺失。免疫组化证实了杂合子突变小鼠新生儿表皮颗粒层中β和γ亚型的缺失。定量RT-PCR显示，P15杂合子突变小鼠新生儿表皮（8倍）、耳朵皮肤（>3倍）和舌（>2倍）中编码α亚型的转录本表达显著增加。（来源：J:214662）
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
