通过CRISPR/Cas9诱变产生了一个30bp的缺失。 Western blot结果显示成年纯合雄性小鼠的睾丸中没有蛋白质表达。(来源:J:254851)
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A 30 bp deletion was generated bu CRISPR/Cas9 mutagenesis. Western blot analysis confirmed the absence of protein expression in the testes of adult homozygous male mice. (J:254851)