这个靶向载体设计用于在exon 1下游插入一个包含增强绿色荧光蛋白(EGFP)序列的盒式元件,带有polyadenylation信号。具体来说,载体从5'至3'包含了FRT引导的诺卡因抗性(neo)座,一个被内向的2272loxP和5171loxP序列环绕的反向EGFP,以及第三个FRT座位于反式EGFP下游。通过Flp介导的重组,FRT引导的neo座被移除。(来源:J:317987)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129
Targeted
插入
--
1
9
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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