使用BACs(细菌人工染色体)构建的靶向载体,通过插入2A-Cre cassette,将P2rx2基因的TGA(stop)(stop)密码子替换,通过SDA(self-deletion anchor)位点去除Neo cassette。这个载体被转染到C57BL/6 ES细胞中,通过同源重组建立小鼠品系,参考文献为(J:290412)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6
Targeted
插入
--
1
2
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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