使用BACs(细菌人工染色体)构建的靶向载体,通过插入2A-Cre cassette,将P2rx2基因的TGA(stop)(stop)密码子替换,通过SDA(self-deletion anchor)位点去除Neo cassette。这个载体被转染到C57BL/6 ES细胞中,通过同源重组建立小鼠品系,参考文献为(J:290412)。
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count