Tg(Ctsl-Gdnf/EGFP)1Reb

A mouse Gdnf cDNA was cloned in-frame with the EGFP from the Pem212 clone, where human MT2A intron 1 was inserted into the EGFP coding region at nucleotide 898. The Pem promoter was then replaced by the Cathepsin L KpnI-SalI promoter/intron 1 fragment from a luciferase reporter construct. One of six characterized transgenic lines was used for further analysis. (J:260462)