Ly6g^{tm2621(cre)Arte}

The Ly6g coding sequence in exon 1 and the splice donor site at the junction of exon 1 and intron 1 were replaced with a cassette containing the open reading frame of Cre recombinase and tdTomato separated by a self-splicing T2A peptide. A human growth hormone polyadenylation signal was inserted at the 3' end of the Cre-T2A-tdTomato sequence in order to prevent downstream transcription of the remaining mouse Ly6g. The FRT flanked positive selection marker (puromycin resistance (PuroR)) was removed after the successful generation of transgenic mice by crossing with Flpe expressing mice. (J:227159)