Rag2^em3Hwl

A p.Arg229Gln mutation was created in the coding region by micro-injecting zygotes with Cas9 mRNA, Scr7, two sgRNAs, and an ssODN (single-stranded oligodeoxynucleotide) containing the desired GC>AG substitution plus three silent substitutions to prevent Cas9 re-cutting. The R229Q mutation is found in some severe combined immunodeficiency (SCID) and Omenn syndrome (OS) patients. (J:276940)