这个来自TCPR0319项目的等位基因是在phenogenomics中心通过注入Cas9 D10A内切酶(nickase)mRNA和四条指导RNA,它们带有TCCACATTTTGCTCGGCTCA和CTGCGTTTTACCTTCCCCAA的 spacer序列,目标是ENMUSE00000514786外显子的5'端,以及GTACAGATTCATGGGCCCAC和GCAGGCGGGTGTGAAGCATC,针对3'端,产生了chr18上一个571bp的删除,范围从77,847,863到77,848,434,以及chr18上一个21bp的缺失,位置在77,848,578到77,848,599,伴随一个insGA插入(GRCm38)。(J:200814)
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This allele from project TCPR0319 was generated at The Centre for Phenogenomics by injecting Cas9 D10A endonuclease (nickase) mRNA and four guide RNAs with spacer sequences of TCCACATTTTGCTCGGCTCA and CTGCGTTTTACCTTCCCCAA targeting the 5' side and GTACAGATTCATGGGCCCAC and GCAGGCGGGTGTGAAGCATC targeting the 3' side of exon ENMUSE00000514786 resulting in a 571 bp deletion of Chr18 from 77847863 to 77848434 and a 21 bp del of Chr18 from 77848578 to 77848599 with insGA (GRCm38). (J:200814)