CRISPR对携带有Thratm1Ffla基因,5'末端有floxed neo cassette插入,这个cDNA编码了L400R氨基酸替换的开放阅读框,以及3'端带有IRES-Tau-lacZ序列的胚胎,导致了从位置393开始的框架移位和在401位获得终止密码子的变异。(来源:J:268664)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count