Slc6a3^{tm1.1(flpo)Fuyu}

The DAT-ires-Flpo targeting construct was designed to insert a loxN-flanked neomycin resistance (neo) cassette followed by an internal ribosomal entry site (IRES) and a Flpo recombinase sequence downstream of the stop codon of the solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3) gene. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to CMV-cre mice to remove the neo cassette. (J:101977, J:340041)