这个来自TCPR0621项目的等位基因是在遗传学中心通过向细胞注射Cas9 mRNA和四条带有特定 Spacer序列(TTCTGACTTGTGTACTCAGG和TTGGAACAGGGCTCACACGT)的guide RNA,分别针对ENSMUSE00000108337基因的5'端和3'端,产生的。这个操作导致了chr11染色体上两个变异:一个2,115bp的删除,起始于80,192,852,终止于80,194,966,插入了'AAGGCA'(GRCm38建库),另一个是390bp的删除,位置在80,195,037到80,195,426。 (J:200814)
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This allele from project TCPR0621 was generated at The Centre for Phenogenomics by injecting Cas9 mRNA and four guide RNAs with spacer sequences of TTCTGACTTGTGTACTCAGG and TTGGAACAGGGCTCACACGT targeting the 5' side and GGCCATTCCCACCCAATCAG and TGATCCCTAATCCTAGTTTA targeting the 3' side of exon ENSMUSE00000108337 resulting in a 2,115-bp deletion of Chr11 from 80192852 to 80194966_insAAGGCA & a 390-bp deletion of Chr11 from 80195037 to 80195426 (GRCm38). (J:200814)