这个来自TCPR0782项目的研究等位基因是在表型遗传学中心通过电穿孔方式,使用了四条带有CGGCCCTCCCTTGTCTAAAA和CGTCACCAGAGGCATAAATG指导RNA,它们的间隔序列分别为5'侧的GGCGCTGCCGACCCATCTTC和3'侧的CCAATCAGACCAGTGGCGCT,针对ENSMUSE00001316074基因的相应位置。这导致了76个碱基对的7号染色体从49,408,593到49,409,268位置的缺失,同时在49,408,480位置插入了一个1个碱基的A(GCRm38建库版本,J:200814)。
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This allele from project TCPR0782 was generated at The Centre for Phenogenomics by electroporating Cas9 RNPs with four guide RNAs with spacer sequences of CGGCCCTCCCTTGTCTAAAA and CGTCACCAGAGGCATAAATG targeting the 5' side and GGCGCTGCCGACCCATCTTC and CCAATCAGACCAGTGGCGCT targeting the 3' side of exon ENSMUSE00001316074 resulting in a 676-bp deletion of Chr7 from 49408593 to 49409268_insT; 1-bp insertion Chr7:49408480_insA (GCRm38). (J:200814)