这个来自TCPR0782项目的等位基因是在遗传学表型组学中心通过电穿孔,使用了含有GCGGCCGCGTGGGCCTCAAT和ATCGGGTCTGGCACCGCTCC这两对引物的Cas9核糖核酸同质体,目标是ENSMUSE00000593055基因的5'端,以及TTTCCCTCTCGCAACTGCAC和GAGCCAACGGTCATCAGAGA这两对针对3'端的指导RNA,实现了7号染色体上一个1,434bp的删除,范围从62,418,584到62,420,017,以及62,418,464到62,418,471的8bp删除(GRCm38建库,J:200814)。
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This allele from project TCPR0782 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with four guide RNAs with spacer sequences of GCGGCCGCGTGGGCCTCAAT and ATCGGGTCTGGCACCGCTCC targeting the 5' side and TTTCCCTCTCGCAACTGCAC and GAGCCAACGGTCATCAGAGA targeting the 3' side of exon ENSMUSE00000593055 resulting in a 1,434-bp deletion of Chr7 from 62418584 to 62420017 and a 8-bp deletion of Chr7 from 62418464 to 62418471 (GRCm38). (J:200814)