这个来自TCPR0328项目,通过TCP通过Cas9 mRNA和一个指导RNA,带有spacer序列CTTGTGAAGCCAGACGCGCT,以及单链寡核苷酸编码点突变的等位基因,通过NHEJ修复产生了。结果在 Chr4:141595354-141595361位置有一个8个碱基的删除(GCGTCTGG)和1个碱基的插入(GRCm38)。(来源:J:200814)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6NCrl
Endonuclease-mediated
插入,基因内删除
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1
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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