这个来自TCPR0837项目的等位基因是在表型遗传学中心通过向细胞注射含有4个指导RNA的Cas9核糖核酸复合物实现的,这些RNA的发夹序列分别为GGACATTTGGAGTATCCAGA和GTGCTCTTGCCGCTTTGAGT,目标是基因的5'端GGTCTTATTCTTATCGACTA,以及3'端的ENSMUSE00001275430和ENSMUSE00001257593的外显子区域。这导致了一个1431bp的缺失,位置在染色体4上,从99,740,974到99,742,404;另外在99,740,914到99,740,932有一个20bp的插入(GRCm38建库,J:200814)。
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This allele from project TCPR0837 was generated at The Centre for Phenogenomics by injecting Cas9 ribonucleoprotein complexes with 4 guide RNAs with spacer sequences of GGACATTTGGAGTATCCAGA and GTGCTCTTGCCGCTTTGAGT targeting the 5' side GGTCTTATTCTTATCGACTA and CCTAGATCATAATATGTAAC targeting the 3' side of exons ENSMUSE00001275430 and ENSMUSE00001257593 resulting in a 1431-bp deletion Chr4: 99740974 to 99742404; 20-bp deletion Chr4:99740914 to 99740932_insCCA; Chr4:99742454_insA (GRCm38). (J:200814)