Cfh^tm1.1Song

A T to A single nucleotide mutation was introduced by site-directed mutagenesis resulting in a tryptophan (W) to arginine (R) mutation at amino acid 1206. An FRT-flanked neomycin selection cassette was inserted upstream of exon 22 and was removed via Flp-mediated recombination. The W1206R change in mouse corresponds to the W1183R mutation found in families with atypical hemolytic uremic syndrome. Western blot analysis of plasma indicates that protein levels are higher (115%) than the normal protein levels in wild-type mice. (J:240426)