Nfix^em2H

CRISPR/Cas9 technology generated an in-frame 24-bp deletion in exon 7 from position +49,561 to +49,584 relative to the translation start site. This deletion causes skipping of exon 7 and alternative splicing of exon 6 to exon 8 to produce wild-type Nfix isoforms. MEFs express the mutant long isoform (293 bp) and the wild-type short isoform lacking exon 7 (194 bp). (J:343087)