Tg(TcraC14,TcrbC14)DUC18Pmal

Valpha10.1 V-J and Vbeta8.3 V-D-J segments were amplified by RT-PCR from the C18 T cell clone. The V-J and V-D-J exon cassettes were cloned into TCR and shuttle vectors, and founder mice were screened by PCR for cointegration of the alpha and beta minigene constructs and by FACS for transgenic TCR expression. One founder was identified. These mice express an H-2Kd -restricted transgenic T cell receptor specific for the syngeneic CMS5 fibrosarcoma rejection antigen mutated ERK2(136-144). (J:271337)