The transposon containing an unspecified hybrid actin promoter driving expression of red fluorescent protein (RFP) flanked by piggyBac-specific left and right terminal sequences was co-injected with a piggyBac-containing transgene to mobilize the transposon. Line 1 inserted into the second intron (Chr:12.11268138, Ensembl release 54). (J:123680, J:272082)