Fgf10^{tm2.1(cre/ERT2)Sbel}

An EGFP gene and inducible cre/ERT2 fusion were inserted into the 3' UTR immediately downstream of the stop codon of the exon 3. The self-cleavage LF2A and T2A sequences will lead to 3 distinct proteins. Flp-mediated recombination removed the selection cassette. The homozygous embryos suffered from lung and limb agenesis and the analysis of Fgf10 expression by qPCR indicated a drastic reduction in Fgf10 expression. It is a loss-of-function allele. (J:308431)