Col1a1^{tm1(CAG-rtTA,tetO-GFP/RNAi:rluc)Hail}

The transgene construct was inserted using recombinase-mediated cassette exchange (RMCE). The transgene construct contains the following elements: the CAG promoter driving the expression of the reverse tetracycline-controlled transactivator, and the tet operon tetracycline responsive element driving the expression of turboGFP green fluorescent protein reporter. The GFP gene is fused with sequence for a miR30-based shRNA targeting Renilla reniformis luciferase. Only upon supplying mice carrying this allele with doxycycline (or tetracycline) will the GFP/shRNA hybrid gene be expressed. (J:274052)