Tg(Myh6-GCaMP8)B4-10Mik

The transgene was designed with (from 5' to 3') a ~5.5 kbp mouse Myh6 minimal promoter sequence (including the 3' most exon of betaMHC, the first three exons of alphaMHC [up to the start codon] as well as intervening sequences) and a ~1950 bp GCaMP8-hGHpA construct. GCaMP8 is composed of (from N-term to C-term) a polyHis plasmid leader sequence essential for thermal stability (RSET with the ΔH and ΔR2 mutations), a 20 residue peptide of chicken smooth muscle myosin light chain kinase (M13; target peptide for a Ca2+-bound CaM), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), a rat calmodulin DNA fragment (CaM; aa 2-148). Founder line B4-10 was identified with high sensor expression in cardiomyocytes. (J:314125)