Cenpf^em2Bko

CRISPR/Cas9 technology generated a 2 bp deletion with a 4 bp insertion at the very beginning of exon 17 resulting in a frameshift starting from residue G2871 which is expected to result in truncation of the last two exons which encode the C-terminal microtubule-binding domain and the farnesylation motif. Western blot analysis with an antibody against the C-terminus confirmed that the C-terminus is missing. Western blot with an antibody against an epitope upstream of the truncation show reduced protein levels. However, quantitative RT-PCR did not detect differences in mRNA levels, indicating that protein is destabilized. (J:273098)